Efeito de uma bebida funcional à base de farinha de banana (Musa spp.) verde sobre a microbiota intestinal, níveis de citocinas e capacidade antioxidante em mulheres com excesso de peso e adiposidade abdominal

Abstract

Objective: To evaluate the effect of a drink unripe banana flour base (UBF) with cocoa in intestinal microbiota, cytokine levels and total antioxidant capacity in women with overweight and abdominal obesity. Methodology: a randomized prospective double-blind clinical trial, conducted from July 2014 to November 2014. The study was conducted with registered volunteers in Nourishing Lives Project, aged 20 to 50 years old. They were randomly divided into two groups of 30 volunteers each. The test group received a milk drink with added UBF and the control group received a milk beverage with the same ingredients, but without addition of UBF. To analyze the effect of drink on intestinal microbiota were evaluated consistency, color, shape and determination of short chain fatty acids (SCFA) in the feces of volunteers. And yet a questionnaire based on Gastrointestinal Sympton Rating Scale (GSRS). The determination of SCFA was made by high-performance liquid chromatography (HPLC). The quantification of cytokines was performed using the cytometric bead array kit (CBA). And for assessing total antioxidant capacity (TAC) an enzymatic kit was used. Data from the study were analyzed using the SPSS software version 19. For consistency and color of faeces was used Fisher's exact test. Applied the T-Test paired for analysis of SCFA and CAT. For GSRS Questionnaire and cytokines used the Wilcoxon test for variables and nonparametric Mann-Whitney test to compare the effect between treatments. The significance level was set at p <0.05. Results: The drink to UBF presented in a high-fiber composition, resistant starch and phenolic compounds. No significant effect on the color and consistency of faeces. However, it promoted increase of propionic acid and reducing gastrointestinal symptoms. Also has influence on the immune system with increased interleukin-2 (IL-2) after the intervention study. No effect on TAC was observed with the consumption of the product developed. Conclusion: The functional beverage to UBF base exerts considerable effect on the intestinal microbiota and IL-2. However there was no observable effect on TAC.