Avaliação de Fitoquímicos e das Atividades Antioxidante Celular e Antiproliferativa do Suco de Araçá-Una (Psidium Eugeniaefolia) e Araçá Morango (Psidium cattleianum var. lucidum).

Abstract

Brazilian native fruits are excellent sources of bioactive compounds that are associated with a lower incidence of chronic diseases such as cancer. The araçá-una (Psidium eugeniaefolia) and araçá morango (Psidium cattleianum var. lucidum) are found in the rainforest and belong to the Myrtaceae family, which has some fruit with biological activities have been studied. This study aimed to determine the total phenolic content and flavonoids, cell antiproliferative and antioxidant activity, and identification of bioactive compounds from extracts of araçá-una e araçá morango. For quantification of the phenolic compounds used the colorimetric method of Folin-Ciocalteu reagent and flavonoids modified colorimetric method. The antioxidant activity was determined by Peroxyl Radical Scavenging Capacity assay (PSC) and cellular antioxidant activity (CAA). Antiproliferative activity of hepatic (HepG2), breast (MCF-7) and lung (A549) carcinoma cells was evaluated by MTS methods and count with trypan blue. The identification of phenolic compounds was performed by high-performace liquid comatografia (HPLC) and nuclear magnetic resonance (NMR). The content of total phenols and flavonoids of araçá morango (463.75 ± 23.68 mgGAE/100g sample and 107.75 ± 29.35 mgCE/100g sample, respectively) were higher than in the araçá-una (252 08 ± 11.46 mgGAE/100 g of sample and 66.51 ± 4.29 mgCE/100g sample, respectively). In tests of the CAA and PSC araçá-una (599.11 ± 3.81 mmol equiv of Vit C/100g and 117.91 ± 14.14 mmol QE/100g, respectively) and araçá morango (482.26 ± 28.36 mmol equiv of Vit C/100g and 98.72 ± 3.14 mmol QE/100g, respectively) showed similar antioxidant activity, which is higher than other fruits have been studied. Decreased the viability of MCF-7 cells treated with 50μg/ml of strawberry guava in the MTS test. The count with trypan blue showed a greater amount of non-viable cells in MCF7 cells treated with 50μg/ml of strawberry guava. In liquid chromatography were not found quercetin, caterquina, kampferol, rutin and apigenin in any of the extracts and the NMR test showed the presence of sugars, which can be linked phenolic compounds. Thus, this study showed that juice of araçá-una and araçá morang are good strategies for detoxification because are sources of phenolic compounds and flavonoids, and show antioxidant activity and in the case of araçá morango antiproliferative activity related to apoptosis of cancer cells.