Investigação da participação espinhal dos receptores TRPV1 na ativação da via PI3K/AKT/mTOR durante a dor pós-operatória em camundongos

Abstract

Approximately 86% of individuals undergoing a type of surgery report experiencing postoperative pain (POP), which is often moderate to severe, starting acutely and may become chronic. Furthermore, few mechanisms have been described in the literature that elucidate the genesis of POP. Given this context, the present study investigated the participation of the transient potential receptor vanilloid type 1 (TRPV1) in the activation of the PI3K/AKT/mTOR pathway in spinal glial cells during POP. Male mice (20-25g) of the C57BL6 lineage were used. The POP model used was the muscle incision and retraction surgery (SMIR) and the nociceptive threshold was assessed using the von Frey filament test. For the pharmacological evaluation of the TRPV1/PI3K/AKT/mTOR pathways and glial cells in POP, the animals received intrathecal blockers and specific inhibitors for TRPV1, PI3K. AKT, mTOR which were administered on the 14th day of POP, followed by assessment of the nociceptive threshold. On the 14th day, animals that received intrathecal administration of the PI3K/AKT/mTOR pathway inhibitors and the TRPV1 blocker showed an increase in the nociceptive threshold. In the biomolecular analysis, an increase in the expression of TRPV1 receptors (p<0.05) and p-mTOR (p<0.05) was observed compared to the naïve group. However, TRPV1 blockade did not alter mTOR expression. According to the results obtained, we suggest the involvement of the PI3K/AKT/mTOR and TRPV1 pathway in the pathophysiology of POP, however the relationship of TRPV1 in the activation of the pathway was not observed.